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Isolation and Study of Temperature-sensitive Mutants of Rabies Virus

¹ Bâtiment 400 Génétique 2 Université de Paris-Sud, 91405 Orsay Cedex, France
² Université Mohammed V Département de Biologie Faculté des Sciences, Rabat, Morocco

One-hundred and seventeen temperature-sensitive (ts) mutants have been isolated from the challenge virus strain (CVS) of rabies virus (RV). The criterion used for this selection was the absence of plaque-forming units on CER cells under agarose medium at the non-permissive temperature (NPT) of 38.5 °C. Of these mutants, 102 were induced by 5-fluorouracil. This compound was much more effective as a mutagen than either nitrous acid or the alkylating agents ethyl methanesulphonate and methyl methanesulphonate. Seventy-six of the ts mutants exhibited residual multiplication at NPT which was less than 2% of that of the wild-type virus. Attempts to demonstrate complementation were unsuccessful, although three phenotypic groups have been established by biochemical and immunological tests. Possible reasons for the failure to obtain complementation are discussed.

Keywords: rabies, ts mutants, mutagen, complementation

Received 30 September 1981; accepted 4 January 1982.