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Effect of a Mutation in the VSV G Protein on the Synthesis and Maturation of Nucleocapsids

Department of Bacteriology and Immunology School of Medicine, University of North Carolina, Chapel Hill, North Carolina 27514, U.S.A.

The tsO45 (V) mutant of vesicular stomatitis virus (VSV) has been used to demonstrate that discrimination between positive (+) and negative (-) strand nucleocapsids in the process of budding mature virions is maintained despite the lack of detectable G protein in either the plasma membrane or the virus membrane. These data indicate that the VSV G protein plays no active role in the discrimination between negative- and positive-strand nucleocapsids which will mature to virions. The synthesis of intracellular nucleocapsids in tsO45 (V)-infected cells at permissive and non-permissive conditions was also examined in comparison with that of the wild-type virus. No differences were observed in intracellular nucleocapsid quantity or polarity in any of these cases despite a 97% inhibition of virus yield in the case of tsO45 (V) at non-permissive temperature.

Keywords: VSV, nucleocapsid, RNA, glycoprotein

Received 6 August 1981; accepted 3 December 1981.