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A Comparison of Paramyxoviruses by Immunoprecipitation

Division of Virology National Institute for Medical Research, Mill Hill, London NW7 1AA, U.K.

Using the technique of immune precipitation of [³⁵S]methionine-labelled infected cell polypeptides followed by SDS-polyacrylamide gel electrophoresis and autoradiography it has been shown that SV5 and a closely related isolate are both antigenically related to human parainfluenza virus type 2. Limited cross-reactions were also demonstrated between parainfluenza virus types 1 and 3 by this method and the apparent molecular weights of the major structural components of human parainfluenza virus types 2 and 3 have been deduced.

Keywords: paramyxoviruses, comparative immunoprecipitation, nucleoproteins

Received 8 October 1981; accepted 14 December 1981.