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Production of High Titres of Interferon- by Cells Derived from Short-term Cultures of Murine Spleen Leukocytes in T-Cell Growth Factor-conditioned Medium

¹ Institute for Virus Research
and² Institute for Immunology and Genetics German Cancer Research Centre, Heidelberg, Federal Republic of Germany

Short-term culture of activated murine spleen cells in T-cell growth factor-conditioned medium resulted in a cell population that produced high titres of interferon- in response to the T-cell mitogen concanavalin A. Compared to interferon- production by freshly isolated spleen cells in response to the same mitogen, titres were 10 to 20 times higher at all cell densities tested. Furthermore, the minimal cell density at which interferon- production could be detected was significantly lower. The mitogen dose required for optimal interferon induction in this system was higher than in conventional spleen cell cultures.

Keywords: spleen cells, T-cell growth factor, IFN-, concanavalin A

Received 21 October 1981; accepted 15 December 1981.