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Ultraviolet-irradiated Vesicular Stomatitis Virus and Defective-interfering Particles are Similar Non-specific Inhibitors of Virus Infection

Department of Physiology and Biophysics, College of Medicine and Dentistry of New Jersey, Rutgers Medical School, Piscataway, New Jersey 08854, U.S.A.

The way in which ultraviolet-irradiated vesicular stomatitis virus (VSV) inhibits the early events in VSV infection has been further characterized. Comparison of several different u.v.-irradiated thermolabile, temperature-sensitive mutants before and after heat inactivation established a requirement for inhibitory activity of functional G, N and L proteins, but not M protein. Defective-interfering (DI) particles, whether irradiated or not, inhibited VSV primary transcription as efficiently as UV-VSV, suggesting that virus proteins rather than transcription products are responsible for inhibition. Addition of inhibitory UV-VSV at different times after infection established that inhibition results from an action at an intracellular site, rather than at the cell surface or in the process of internalization. A similar inhibition by UV-VSV of infection by Sendai virus, Semliki Forest virus, Sindbis virus and influenza virus suggests that UV-VSV is acting by inducing a general change in the intracellular environment.

Keywords: VSV, Sendai virus, SFV, Sindbis virus, influenza virus, cytopathic effects

Received 15 September 1981; accepted 22 January 1982.

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