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Liposome-mediated Infection of Turnip Protoplasts with Turnip Rosette Virus and RNA

F. Rollo

John Innes Institute, Colney Lane, Norwich NR4 7UH, U.K.

Turnip rosette virus (TRosV) and its RNA have been entrapped in multilamellar vesicles (MLVs) composed of phosphatidylcholine and -sitosterol (neutral MLVs) or of phosphatidylcholine and sterylamine (positively charged MLVs). TRosV RNA was also entrapped in vesicles of phosphatidylcholine, -sitosterol and dicetylphosphate prepared by the reverse phase evaporation method (REVs). TRosV in positively charged or neutral MLVs will infect turnip protoplasts in the presence of fusogenic agents (polyethylene glycol and/or CaCl₂); TRosV RNA in such MLVs did not infect protoplasts. TRosV RNA in REVs infected protoplasts whereas the unentrapped RNA did not.

Keywords: turnip rosette virus, liposomes, plant protoplasts, multilamellar vesicles

Present address: Instituto di Microbiologia e Fisiologia Vegetale, via Sant'Epifanio 14, 27100 Pavia, Italy.

Received 9 November 1981; accepted 5 February 1982.