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Selection of Temperature-sensitive Mutants in Persistent Infection by Parainfluenza Virus Type 3

Department of Pediatrics College of Physicians and Surgeons Columbia University, New York, New York 10032, U.S.A.

Vero cells persistently infected with parainfluenza virus type 3 (para 3) were examined for the production of temperature-sensitive (ts) mutants. After 6 months and 33 passages, ts mutants formed the great majority of the virus being shed into the supernatant fluid. Complementation studies gave evidence that the mutants shared a common lesion. Although ts mutants may play a role in the maintenance of persistent infection in this system, the mutants proved to be unstable when removed from the milieu of persistent infection. It is thus possible that their selection may be secondary to (an) unidentified factor(s) which play(s) a more primary role in the maintenance process.

Keywords: parainfluenza virus, ts mutants, persistent infection, paramyxoviruses

Received 11 January 1982; accepted 15 February 1982.