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J Gen Virol 61 (1982), 137-141; DOI 10.1099/0022-1317-61-1-137
© 1982 Society for General Microbiology
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The Effect of Urea on Tobacco Mosaic Virus: Polarity of Disassembly

L. E. Blowers and T. M. A. Wilson

Department of Biochemistry University of Liverpool, P.O. Box 147, Liverpool L69 3BX, U.K.

Urea acts to disassemble tobacco mosaic virus (TMV) predominantly from the 5'-end of the virus RNA. Extensively uncoated particles exhibit some degree of bi-directional uncoating as judged by a reduced ability to bind [3H]histidine. The translational activities of the RNA fragments which remained encapsidated in the urea-resistant fraction of TMV rods were investigated.

Keywords: TMV, urea stripping, polarity, translational activity

Received 3 November 1981; accepted 18 February 1982.




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