HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Toriyama, S. Search for Related Content PubMed Articles by Toriyama, S. Agricola Articles by Toriyama, S.

Characterization of Rice Stripe Virus: a Heavy Component Carrying Infectivity

Laboratory of Plant Pathology, Faculty of Agriculture, University of Tokyo, Bunkyo-ku, Tokyo 113, Japan

Rice stripe virus (RSV) preparations contain a previously unreported nucleoprotein component (nB) which sediments faster in sucrose gradients than middle (M) and bottom (B) components; the latter two components are 20-fold more concentrated than nB. Electron microscopy of purified nB preparations revealed filamentous particles (8 nm wide), and other structures which might be derived from these particles. The nB preparations contained four RNA species of mol. wt. 0.9 x 10⁶, 1.0 x 10⁶, 1.4 x 10⁶ and 1.9 x 10⁶ (2700, 3000, 4100 and 5600 nucleotides respectively); the three smaller RNAs may be derived from M and B components contaminating nB. A single protein (mol. wt. 3.2 x 10⁴) was present in M, B and nB preparations. The nB component appears to be required for infectivity because the planthopper vector (Laodelphax striatellus) became viruliferous after injection with nB but not after injection with M or B.

Keywords: rice stripe virus, purification, electron microscopy, infectivity

Received 5 January 1982; accepted 5 March 1982.