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Genomic Location and Lack of Phosphorylation of the HSV Immediate-Early Polypeptide IE 12

Medical Research Council Virology Unit, Institute of Virology, University of Glasgow, Church Street, Glasgow G11 5JR, U.K.

Herpes simplex virus type 1 (HSV-1) induces an immediate-early (IE) polypeptide IE 12. An equivalent polypeptide coded by HSV-2 which migrated slightly more slowly on SDS-polyacrylamide gels was identified and designated IE 12.3. Analysis of the serotype of the IE polypeptide induced by five HSV-1/HSV-2 intertypic recombinants when correlated with their genome structures showed that IE 12/IE 12.3 mapped in the region spanning the TR_S/U_S junction. Unlike four other IE polypeptides induced by HSV-1 (IEs 175, 110, 68 and 63), IE 12 was not detectably phosphorylated.

Keywords: HSV, phosphoproteins, immediate-early proteins, gene mapping

Received 20 January 1981; accepted 26 March 1982.