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A Covalently Linked Protein at the 5'-Ends of the Genomic RNAs of Pea Enation Mosaic Virus

¹ Department of Molecular Biology
and² Department of Plant Pathology University of Wisconsin-Madison, Madison, Wisconsin 53706, U.S.A.

Evidence is presented for the presence of a covalently linked protein that is attached to the 5'-ends of genomic RNA of pea enation mosaic virus (PEMV). Nuclease digestion of PEMV RNA released protein with an apparent mol. wt. of approx. 17500. The absence of a 5'-cap and the inability to introduce [³²P]phosphate into the 5'-termini of these RNAs suggests that the protein is covalently bound to PEMV RNA.

Keywords: PEMV, genomic RNAs, 5'-end, covalently linked protein

Received 21 January 1982; accepted 22 April 1982.