HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Harnett, G. B. Articles by Shellam, G. R. Search for Related Content PubMed PubMed Citation Articles by Harnett, G. B. Articles by Shellam, G. R. Agricola Articles by Harnett, G. B. Articles by Shellam, G. R.

Variation in Murine Cytomegalovirus Replication in Fibroblasts from Different Mouse Strains in vitro: Correlation with in vivo Resistance

¹ Virus Laboratory, State Health Laboratory Service of Western Australia
and² Department of Microbiology, University of Western Australia, Queen Elizabeth II Medical Centre, Nedlands, 6009, Western Australia

The in vitro growth of murine cytomegalovirus (MCMV) in mouse embryo fibroblasts (MEF) from Balb/c, C57BL, C3H and CBA mouse strains has been shown to correlate with the genetically determined in vivo susceptibility of these strains to MCMV infection. MEF from Balb/c and C57BL mice were more susceptible to MCMV than those from C3H and CBA mice. This was evident regardless of whether replication was measured by cytopathic effect (c.p.e.) score, virus yield, plaque count, plaque size or time of onset of c.p.e. The growth of MCMV in tracheal organ cultures from different mouse strains was similar to that observed in MEF from these strains. The replication in MEF when measured by c.p.e. score and virus yield was affected by the density of the cell cultures. The strain of mouse used to produce MCMV also affected the comparative sensitivity of MEF to the virus. This appeared to be due to reduced growth of MCMV in its homologous MEF type, an unexpected result. In contrast, cell density had little effect on the replication of herpes simplex virus type 1 (HSV-1), but again the in vitro susceptibility of MEF reflected the in vivo susceptibility of mouse strains to infection with this virus.

Keywords: MCMV, fibroblasts, genetic resistance

Received 7 January 1982; accepted 19 April 1982.

This article has been cited by other articles:

				X. Xie, A. Dighe, P. Clark, P. Sabastian, S. Buss, and M. G. Brown Deficient Major Histocompatibility Complex-Linked Innate Murine Cytomegalovirus Immunity in MA/My.L-H2b Mice and Viral Downregulation of H-2k Class I Proteins J. Virol., January 1, 2007; 81(1): 229 - 236. [Abstract] [Full Text] [PDF]

				R. Holtappels, J. Podlech, G. Geginat, H.-P. Steffens, D. Thomas, and M. J. Reddehase Control of Murine Cytomegalovirus in the Lungs: Relative but Not Absolute Immunodominance of the Immediate-Early 1 Nonapeptide during the Antiviral Cytolytic T-Lymphocyte Response in Pulmonary Infiltrates J. Virol., September 1, 1998; 72(9): 7201 - 7212. [Abstract] [Full Text] [PDF]