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J Gen Virol 62 (1982), 219-225; DOI 10.1099/0022-1317-62-2-219
© 1982 Society for General Microbiology
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Biochemical Analysis and Electron Microscopic Study on Intracellular Virions in NIH/3T3 Mouse Cells Chronically Infected with Moloney Murine Leukaemia Virus: Effect of Interferon

Mordechai Aboud1, Ruth Shoor2, Sara Bari2, Yehudith Hasan1, Revital Shurtz2, Zvi Malik2 and Samuel Salzberg2

1 Unit of Microbiology and Immunology, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel
and2 Department of Life Sciences, Bar-Ilan University, Ramat-Gan 52 100, Israel

Radioactively labelled virus particles of intracellular origin were isolated from the cytoplasmic fraction of disrupted NIH/3T3 cells chronically infected with Moloney murine leukaemia virus [NIH/3T3 (MLV)]. Interferon (IFN) treatment for 48 h, which arrested more than 90% of virus release, resulted in a remarkable accumulation of these intracellular virions. However, no major effect of such treatment was apparent on their structural properties. Transmission electron microscopic examination revealed that these intracellular virions were located within cytoplasmic vacuoles. IFN treatment resulted in a considerable increase in the number of virus-containing vacuoles, as well as the total number of vacuolar virions. It seems that IFN inhibits the final release of vacuolar virions from the cells, thus leading to their intracellular accumulation.

Keywords: M-MLV, interferon, biochemical analysis, electron microscopy

Received 13 January 1982; accepted 26 April 1982.




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Copyright © 1982 by the Society for General Microbiology.