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J Gen Virol 62 (1982), 313-322; DOI 10.1099/0022-1317-62-2-313
© 1982 Society for General Microbiology
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Isolation of a Bisegmented Double-stranded RNA Virus from Thirlmere Reservoir

D. C. Kelly1, M. D. Ayres1, S. C. Howard1, T. Lescott1, M. K. Arnold1, N. D. Seeley2 and S. B. Primrose2,{dagger}

1 Natural Environment Research Council, Institute of Virology, Mansfield Road, Oxford OX1 3SR, U.K.
and2 Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, U.K.

A novel bisegmented double-stranded RNA virus has been isolated from water processed from Thirlmere reservoir. The virus is icosahedral, 58 nm in diam., has a buoyant density of 1.32 g/ml in CsCl, has an S value of 400 and a RNA/protein ratio of 0.087. The two linear segments of RNA have approx. mol. wt. of 2.26 x 106 and 2.09 x 106. The virus contains six polypeptides. The virus was isolated in Drosophila melanogaster cells and fails to replicate in other insect, amphibian, avian, piscine, mammalian and plant cells tested. The virus is biochemically different from infectious pancreatic necrosis virus (IPNV) and Drosophila X virus (DXV). The virus is also serologically unrelated to IPNV (strain Sp) and another invertebrate pathogenic virus, Tellina virus 1. The virus shares common antigens with DXV but is not completely identical.

Keywords: birnavirus, double-stranded RNA, bisegmented genome

{dagger} Present address: Searle Research and Development, Division of G.D. Searle & Co. Ltd., P.O. Box 53, Lane End Road, High Wycombe, Bucks., HP12 4HL, U.K.

Received 15 February 1982; accepted 20 April 1982.




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Copyright © 1982 by the Society for General Microbiology.