HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Brown, D. A. Articles by Bignell, G. N. Search for Related Content PubMed PubMed Citation Articles by Brown, D. A. Articles by Bignell, G. N. Agricola Articles by Brown, D. A. Articles by Bignell, G. N.

The Use of a Protein A Conjugate in an Indirect Enzyme-linked Immunosorbent Assay (ELISA) of Four Closely Related Baculoviruses from Spodoptera Species

Natural Environment Research Council Institute of Virology, Mansfield Road, Oxford OX1 3SR, U.K.

The virus particles from four closely related insect nuclear polyhedrosis virus (NPVs) isolated from their homologous Spodoptera spp. hosts were examined using an indirect enzyme-linked immunosorbent assay (ELISA), employing both swine anti-rabbit and protein A conjugate. Results from both systems indicated that S. littoralis NPV is serologically distinct from the other three viruses, S. frugiperda NPV, S. exempta NPV and S. exigua NPV, which are closely related. The relatedness of these viruses is discussed with reference to existing knowledge and the results obtained from both conjugate systems.

Keywords: baculovirus, protein A, ELISA

Received 23 February 1982; accepted 24 May 1982.