HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Ghendon, Y. Z. Articles by Hay, A. J. Search for Related Content PubMed PubMed Citation Articles by Ghendon, Y. Z. Articles by Hay, A. J. Agricola Articles by Ghendon, Y. Z. Articles by Hay, A. J.

Studies of Fowl Plague Virus Temperature-sensitive Mutants with Defects in Transcription

Research Institute for Viral Preparations, Moscow, U.S.S.R.
and¹ National Institute for Medical Research, Mill Hill, London NW7 1AA, U.K.

Two fowl plague virus temperature-sensitive (ts) mutants belonging to different complementation groups were studied. Both were defective in the syntheses of unpolyadenylated complementary RNA [A(-)cRNA] and virus RNA (vRNA) at non-permissive temperature whereas primary transcription was unaffected. In addition, ts 29, in which the ts mutation is in gene 1 coding for polypeptide P₃, has a defect in ‘secondary’ synthesis of mRNA at non-permissive temperature whereas inhibition of mRNA synthesis by ts 131, in which the ts mutation is in gene 2 coding for polypeptide P₁, appeared to result from a defect in vRNA synthesis. These results indicate, therefore, that different enzymes are responsible for the syntheses of virus mRNAs and A(-)cRNAs, which is consistent with the apparent differences in initiation and termination of transcription in the two reactions. The patterns of synthesis of the various types of virus RNA during infection are discussed.

Keywords: influenza virus, ts mutants, transcription

Received 10 March 1982; accepted 21 June 1982.