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Micromonas pusilla Virus: the Virus Growth Cycle and Associated Physiological Events Within the Host Cells; Host Range Mutation

Department of Oceanography, The University of British Columbia, Vancouver, British Columbia, Canada, V6T 1W5

The photosynthetic marine flagellate Micromonas pusilla (Butch.) Manton et Parke (Prasinophyceae) and its cytoplasmic virus, M. pusilla virus (MPV), were cloned. Host cells were maintained in liquid culture. Infectivity titration was by endpoint dilution, using loss of host chlorophyll as an indicator of the presence of infective virus. The virus growth cycle was characterized by an eclipse period of 3 h, a latent period of 7 h and a total lytic cycle of 14 h. The average burst size was 72 infective particles per cell. Inhibition of CO₂ photoassimilation began 2 h after inoculation with virus. An almost immediate decrease in in vivo chlorophyll a fluorescence in infected cells was light-dependent. M. pusilla cells can mutate to virus resistance at the cell surface. Host range mutants of MPV exhibited variable infectivity in different strains of M. pusilla.

Keywords: MPV growth cycle, host cell physiology, host mutation

Received 5 March 1982; accepted 8 June 1982.