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Nucleic Acid-binding Properties of Adenovirus Structural Polypeptides

Division of Virology, National Institute for Medical Research, Mill Hill, London NW7 1AA, U.K.

The ability of adenovirus structural polypeptides to bind nucleic acids was assessed by separating the polypeptides on SDS-polyacrylamide gels, transferring them electrophoretically to nitrocellulose and probing with ³²P-labelled nucleic acids. Polypeptides IVa₂, V, VI and VII, as well as trace amounts of pVII and a polypeptide of apparent mol. wt. 40 x 10³ were able to bind label under these conditions. Labelling was also detected with a smaller polypeptide, possibly related to the cleavage products of pVII and/or pVI. The binding of DNA to polypeptide VI appeared to be more sensitive to detergents than the others. No sequence specificity could be detected in the DNA binding.

Keywords: Adenovirus structural polypeptides, radiolabelled probes, electrophoretic blotting

Received 27 April 1982; accepted 14 June 1982.

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