J Gen Virol Faster Access
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Gen Virol 63 (1982), 255-264; DOI 10.1099/0022-1317-63-2-255
© 1982 Society for General Microbiology
This Article
Right arrow Full Text (PDF)
Right arrow Corrigendum (v64,p759)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Campo, M. S.
Right arrow Articles by Coggins, L. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Campo, M. S.
Right arrow Articles by Coggins, L. W.
Agricola
Right arrow Articles by Campo, M. S.
Right arrow Articles by Coggins, L. W.

Molecular Cloning of Bovine Papillomavirus Genomes and Comparison of Their Sequence Homologies by Heteroduplex Mapping

M. Saveria Campo and Lesley W. Coggins

Beatson Institute for Cancer Research, Wolfson Laboratory for Molecular Pathology, Garscube Estate, Bearsden, Glasgow G61 1BD, U.K.

The genomic DNAs of bovine papillomavirus (BPV) type 1, type 2 and type 4 were cloned in pAT153. BPV1 and BPV2 genomes were cloned using the single HindIII sites of the vector and virus DNAs, and BPV4 was cloned using the single BamHI sites. The orientation of the recombinant DNAs was established by restriction enzyme digestion, hybridization and heteroduplex analysis. The results showed that: (i) BPV1 and BPV2 DNAs are in register and are broadly homologous throughout most of their length when aligned at their single HindIII sites; (ii) depending on the degree of hybridization stringency used, the two DNAs show one major region and several minor regions of partial homology, mainly residing in the segment of the genomes believed to contain the structural genes; (iii) BPV4 DNA shares no homology with either BPV1 or BPV2 DNA.

Keywords: BPV, molecular cloning, heteroduplex and restriction enzyme mapping

Received 21 May 1982; accepted 30 June 1982.


This article has been cited by other articles:


Home page
 J. Gen. Virol.Home page
T. Ogawa, Y. Tomita, M. Okada, and H. Shirasawa
Complete genome and phylogenetic position of bovine papillomavirus type 7
J. Gen. Virol., July 1, 2007; 88(7): 1934 - 1938.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Virol.Home page
T. Ogawa, Y. Tomita, M. Okada, K. Shinozaki, H. Kubonoya, I. Kaiho, and H. Shirasawa
Broad-spectrum detection of papillomaviruses in bovine teat papillomas and healthy teat skin
J. Gen. Virol., August 1, 2004; 85(8): 2191 - 2197.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Virol.Home page
A. Narechania, M. Terai, Z. Chen, R. DeSalle, and R. D. Burk
Lack of the canonical pRB-binding domain in the E7 ORF of artiodactyl papillomaviruses is associated with the development of fibropapillomas
J. Gen. Virol., May 1, 2004; 85(5): 1243 - 1250.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
R.G. Beniston, I.M. Morgan, V. O'Brien, and M.S. Campo
Quercetin, E7 and p53 in papillomavirus oncogenic cell transformation
Carcinogenesis, July 1, 2001; 22(7): 1069 - 1076.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 1982 by the Society for General Microbiology.