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Enhancement of Plasminogen Activator Activity by the Culture Medium of Rous Sarcoma Virus-transformed Cells

Istituto di Genetica, Università di Pavia and Istituto di Genetica, Biochimica ed Evoluzionistica del CNR di Pavia, Via S. Epifanio, 14, 27100 Pavia, Italy

We have tested the effect of the culture medium of chicken embryo fibroblasts (CEF) transformed by Rous sarcoma virus (RSV) on plasminogen activator (PA) activity of normal and RSV-infected cells. The results obtained showed that fibrin digestion by cultures of normal uninfected and RSV-infected cells directly attached to a fibrin substrate was increased when the cells were exposed to the supernatant of an 18 h-culture of CEF transformed by RSV. Addition of cycloheximide to the supernatant completely abolished this effect. Similarly, exposure of both normal and RSV-infected cells to the culture medium of RSV-transformed CEF resulted in a marked increase of the soluble PA activity present in the supernatant. This effect, however, was not abolished by inhibition of protein synthesis. For both the cell-bound and soluble PA activities of normal and RSV-infected cells the stimulatory effect was transient, being maximal between 8 and 12 h of exposure to the transformed cell-conditioned medium and disappearing after 24 h. A possible correlation with transformation-enhancing factor(s), previously reported to be present in the culture medium of transformed cells, is discussed.

Keywords: Rous sarcoma virus, plasminogen activator, transformed cells, transformation-enhancing factors

Received 8 March 1982; accepted 13 July 1982.