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J Gen Virol 63 (1982), 383-391; DOI 10.1099/0022-1317-63-2-383
© 1982 Society for General Microbiology
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African Swine Fever Virus DNA: Restriction Endonuclease Cleavage Patterns of Wild-type, Vero Cell-adapted and Plaque-purified Virus

Ronald D. Wesley and I. C. Pan

United States Department of Agriculture, Agricultural Research Service, Plum Island Animal Disease Center, P.O. Box 848, Greenport, New York 11944, U.S.A.

DNA from African swine fever (ASF) virus was isolated and was characterized by two restriction enzymes, SmaI and EcoRI. Although both enzymes can distinguish Vero cell-adapted ASF isolates by characteristic restriction endonuclease cleavage patterns, all ASF isolates examined exhibited a high degree of similarity, as measured by co-migration of most of the DNA fragments. The molecular weight of ASF DNA, based on size estimates of DNA fragments from cleavage patterns, ranged from 93 x 106 to 100 x 106. Virus genome heterogeneity was observed in uncloned, cell culture-adapted ASF isolates as well as in a plaque-purified virus after serial passage in Vero cells. In contrast to the rather minor differences in restriction pattern among the Vero cell-adapted isolates, a major alteration in restriction endonuclease cleavage sites was observed during adaptation of the wild-type virus to cell culture.

Keywords: African swine fever virus, restriction endonuclease analysis, genome heterogeneity

Received 30 March 1982; accepted 6 July 1982.


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