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Specificity of IgM Antibodies in Acute Human Coxsackievirus B Infections, Analysed by Indirect Solid Phase Enzyme Immunoassay and Immunoblot Technique

Institute of Virology and Immunobiology, University of Würzburg, Versbacher Strasse 7, D-8700 Würzburg, Federal Republic of Germany

The specificity of the IgM response in acute human coxsackievirus B infections was examined by indirect solid phase enzyme immunoassay and immunoblot techniques. IgM antibodies detected by ELISA were either strictly type-specific, type-predominant or group-reactive to coxsackieviruses B-1 to B-5. Homotypic and type-dominant responses were clearly correlated with the serotype isolated from the patient. Analysis of ELISA antigens by SDS-gel electrophoresis, protein transfer and subsequent immunodetection of individual virus polypeptides, revealed VP1 as the major antigen; it was detected by homotypic as well as heterotypic serum specimens.

Keywords: coxsackievirus B, immunoglobulin M response, ELISA, protein-blot

Received 6 April 1982; accepted 17 August 1982.