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Effect of Multiplicity of Infection on Transcription in Escherichia coli Cells Infected by Bacteriophage Lambda

Department of Biology, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong

The effect of multiplicity of infection (m.o.i.) on transcription was studied by infecting Escherichia coli with bacteriophage cI47, cI47O29P3 and cI857cro27P3. DNA-RNA hybridization with cI47 l-strand DNA, 80imm r-strand DNA, and imm80 r-strand DNA were used to measure mRNA transcription from the l-strand, the r-strand of the early x-P-Q region and the late A-J-b2 region of bacteriophage respectively. In cI47cro⁺O^-P^--infected cells, transcription from the l-strand, r-strand of the early x-P-Q region and the late A-J-b2 region all decreased with increasing m.o.i. The response in the x-P-Q region was less marked than in other regions, but the pattern looked similar to that described above. When phage DNA replication was permitted, as in the case of cI47, the response was similar to that observed in cI47cro⁺O^-P^--infected cells, but the level of transcription was increased two- or three-fold. In cI857cro^-P^--infected cells, the leftward transcription and the rightward transcription from the early x-P-Q region and the late A-J-b2 region all increased with increasing m.o.i., but the extent of change was less drastic than with cro⁺. This result demonstrated clearly that the decreased in transcription from various regions at increasing m.o.i. of cro⁺ was due to the inhibitory action of the cro gene product. The results obtained with cro^- strongly support the view that gene dosage is a significant controlling factor for the extent of gene expression.

Keywords: phage lambda, gene dosage, transcription, cro gene

Received 26 June 1981; accepted 16 July 1982.

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