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Either Orientation of the L Segment of the Herpes Simplex Virus Genome May Participate in the Production of Viable Intertypic Recombinants

N. M. Wilkie

MRC Virology Unit, Institute of Virology, University of Glasgow, Church Street, Glasgow G11 5JR, U.K.

We have analysed the genome structures of 90 recombinant viruses produced by co-infection of baby hamster kidney cells with the DNA of a herpes simplex virus type 1 temperature-sensitive mutant (tsD) and specific DNA fragments of wild-type herpes simplex virus type 2 at non-permissive temperature. Crossovers were located predominantly in regions of greatest intertypic homology, and we conclude that primary recombination occurred with the L segment of the genome in either orientation.

Keywords: HSV, recombination, homology, inversion

Present address: The Beatson Institute for Cancer Research, Wolfson Laboratory for Molecular Pathology, Garscube Estate, Switchback Road, Glasgow G61 1BD, U.K.

Received 2 August 1982; accepted 27 August 1982.