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Comparison of the Physical Maps of the DNAs of Two Cytomegalovirus Strains

Laboratorium voor de Gezondheidsleer, Universiteit van Amsterdam, Mauritskade 57, 1092 AD Amsterdam, The Netherlands

The physical maps of the DNAs of two cytomegalovirus isolates, AD169 and SG, were compared by cross-blot hybridization and by hybridization of nitrocellulose-bound SG fragments with cloned ³²P-labelled AD169 fragments. From this comparison it can be concluded that both physical maps are co-linear to a large extent. Most variability existed at the termini of the long and short component (at the repeats). Other differences were the presence or absence of restriction endonuclease cleavage sites.

Keywords: HCMV, DNA, restriction endonuclease, structure

Received 2 February 1982; accepted 17 August 1982.