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J Gen Virol 64 (1983), 83-94; DOI 10.1099/0022-1317-64-1-83
© 1983 Society for General Microbiology
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Virus Gene Expression in Rat Cells Transformed by Avian Myelocytomatosis Virus Strain MC29 and Avian Erythroblastosis Virus

Kristina Quade1,{dagger}, Simon Saule2, Dominique Stéhelin2, Gay Kitchener1 and Michael J. Hayman1

1 Imperial Cancer Research Fund, P.O. Box 123, Lincoln's Inn Fields, London WC2A 3PX, U.K.
and2 INSERM U186, Institut de Pasteur, 59019 Lille, France

Virus gene expression in rat cells transformed by either avian myelocytomatosis virus strain MC29 or avian erythroblastosis virus has been studied by biological and biochemical methods. In the clones examined, virus-specific sequences were found to be transcribed into RNA and, in most clones, the characteristic gag-related proteins could be identified. The transformed rat cells were fused to permissive chick cells and the rescued virus was shown to transform both chick embryo fibroblasts and the appropriate haemopoietic cell type in chick bone marrow cultures. These results clearly demonstrate that, as with the non-defective avian sarcoma viruses, the genetic information responsible for transformation by the defective avian leukaemia viruses can be expressed in non-permissive mammalian host cells as well as in permissive avian cells.

Keywords: avian myelocytomatosis virus, avian erythroblastosis virus, transformed rat cells

{dagger} Present address: Division of Virology, National Institute for Medical Research, Mill Hill, London NW7 1AA, U.K.

Received 18 February 1982; accepted 21 July 1982.




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Copyright © 1983 by the Society for General Microbiology.