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J Gen Virol 64 (1983), 2299-2303; DOI 10.1099/0022-1317-64-10-2299
© 1983 Society for General Microbiology
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Defective Hepatitis B Virus DNA Molecules Detected in a Stable Integration Pattern in a Hepatoma Cell Line, and in Induced Tumours and Derived Cell Lines

J. P. Monjardino, M. J. F. Fowler and H. C. Thomas1

Laboratory of Cell Studies (Department of Physiology)
and1 Academic Department of Medicine, Royal Free Hospital School of Medicine, Rowland Hill Street, London NW3 2PF, U.K.

Hepatitis B virus (HBV) DNA was found to be integrated into seven sites in the DNA of the PLC/PRF/5 hepatoma cell line as determined by digestion with the restriction endonuclease HindIII which does not cut through the viral genome. The integration pattern was stable in the cell line, in tumours induced in athymic mice by this line and in cell lines derived from such tumours. Syntheses of hepatitis B surface antigen and alphafoetoprotein were maintained in the induced tumours and derived cell lines. A defective HBV DNA molecule (approx. 2.8 kilobase pairs) appears to be integrated in a head-to-tail tandem arrangement and it is proposed that such defective molecules may be involved in the process of neoplastic transformation by HBV.

Keywords: HBV, hepatocellular carcinoma, DNA integration, HBsAg

Received 24 March 1983; accepted 28 June 1983.


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 Annals of Clinical & Laboratory ScienceHome page
R. B. Birrer, D. Birrer, and J. V. Klavins
Hepatocellular Carcinoma and Hepatitis Virus
Ann. Clin. Lab. Sci., January 1, 2003; 33(1): 39 - 54.
[Abstract] [Full Text] [PDF]


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