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Co-purification of Protein Kinase Activity with the 58000 Dalton Polypeptide Coded for by the Early 1B Region of Human Adenovirus Type 5

Department of Pathology, McMaster University, Hamilton, Ontario, Canada

Extracts from human adenovirus type 5 (Ad5)-transformed cells were fractionated by ammonium sulphate precipitation, DEAE-Sephacel chromatography and glycerol gradient centrifugation. In all cases, protein kinase activity co-purified with the 58000 mol. wt. polypeptide (58K) coded for by the early 1B region of Ad5. Kinase activity was also precipitated by an antiserum raised against a synthetic peptide corresponding to the predicted carboxy terminus of 58K. These data suggest that protein kinase activity is associated with 58K, either intrinsically or in an enzyme bound to 58K.

Keywords: Ad5, early protein, 58K protein, protein kinase activity

Received 20 April 1983; accepted 26 June 1983.