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J Gen Virol 64 (1983), 2317-2321; DOI 10.1099/0022-1317-64-10-2317
© 1983 Society for General Microbiology
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Antiviral Activities of Cloned Human Leukocyte Interferons against Herpes Simplex Virus Type 2 Infections of Mice

E. N. Fish, K. Banerjee and N. Stebbing1

Division of Infectious Diseases, Research Institute Hospital for Sick Children, 555 University Avenue, Toronto, Ontario M5G 1X8, Canada
and1 Applied Molecular Genetics, Inc., 1900 Oak Terrace Lane, Thousand Oaks, California 91320, U.S.A.

Human {alpha}-interferons (IFN-{alpha}s) made in bacteria were examined for antiviral activity against herpes simplex virus type 2 (HSV-2) infections of mouse L-cells in vitro, and acute cervicovaginal and lethal systemic HSV-2 infections of BALB/c mice. The recombinant DNA-derived hybrid interferon IFN-{alpha}AD(Bgl) showed pronounced antiviral activity in vitro, exceeding the activity of either of the parental subtypes IFN-{alpha}A and IFN-{alpha}D and that of the other hybrids IFN-{alpha}AD(Pvu). A combination of topical and systemic treatments with IFN-{alpha}A and IFN-{alpha}AD(Bgl) failed to protect mice from subsequent challenge with an acute cervicovaginal infection of HSV-2. Protection from lethal systemic HSV-2 infection in mice was observed when IFN-{alpha}AD(Bgl) and IFN-{alpha}AD(Pvu) were administered systemically, whereas IFN-{alpha}A failed to confer protection. These results suggest that for protection against infection with HSV-2, the routes of introduction of the virus and of the interferon influence the host response to interferon therapy.

Keywords: interferon (HuIFN-{alpha}), cloned IFN, HSV-2, antiviral activities

Received 8 March 1983; accepted 10 June 1983.




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Copyright © 1983 by the Society for General Microbiology.