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Protein Synthesis in Semliki Forest Virus-infected Cells is Not Controlled by Permeability Changes

Department of Biochemistry, St George's Hospital Medical School, Cranmer Terrace, London SW17 0RE, U.K.

The uptake of the GTP analogue guanylyl(,-methylene)diphosphonate (GppCH₂p) is the same in Semliki Forest virus (SFV)-infected BHK cells as in mock-infected cells, in spite of the fact that protein synthesis is inhibited by GppCH₂p more markedly in SFV-infected cells than in control cells. A possible explanation for this difference is that infected cells have a lower concentration of GTP and a lower ratio of GTP:GDP than uninfected cells, and the analogue may thus be a more effective competitive inhibitor of translation. We conclude that in this system, the difference between infected and uninfected cells lies not at the plasma membrane but within the cytoplasm.

Keywords: Semliki Forest virus, host cell shut-off, cell permeability

Received 6 June 1983; accepted 15 August 1983.