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Infection of Protoplasts from Several Brassica Species with Cauliflower Mosaic Virus Following Inoculation Using Polyethylene Glycol

John Innes Institute, Colney Lane, Norwich NR4 7UH, U.K.

Using a polyethylene glycol-mediated uptake procedure, protoplasts from a range of Cruciferous species were infected with cauliflower mosaic virus; they include Moricandia arvensis, a plant not known to be susceptible. With this technique >90% of turnip protoplasts were infected, each producing an average of 10⁴ to 10⁵ viral genome equivalents. Filter hybridization and fluorescent antibody staining showed that virus synthesis in turnip protoplasts reaches a maximum 65 to 96 h post-inoculation. In protoplasts from some other species the time course was different; viral DNA synthesis in rape reached a maximum 24 to 48 h post-inoculation, approximately 48 h before the maximum accumulation of viral antigen.

Keywords: CaMV, DNA synthesis, Brassica protoplast, virus synthesis

Received 23 May 1983; accepted 19 August 1983.