J Gen Virol Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Gen Virol 64 (1983), 2749-2761; DOI 10.1099/0022-1317-64-12-2749
© 1983 Society for General Microbiology
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Weder, D.
Right arrow Articles by Radsak, K. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Weder, D.
Right arrow Articles by Radsak, K. D.
Agricola
Right arrow Articles by Weder, D.
Right arrow Articles by Radsak, K. D.

Induction of a Host-specific Chromatin-associated Glycopolypeptide by Human Cytomegalovirus

Dagmar Weder and Klaus D. Radsak

Zentrum für Hygiene und Medizinische Mikrobiologie der Universität Marburg, Pilgrimstein 2, 3550 Marburg, Federal Republic of Germany

Analysis of chromatin preparations from [3H]glucosamine-labelled human foreskin fibroblasts revealed that a chromatin-associated glycopolypeptide with the approximate mol. wt. 130000 (130K) is induced in response to either infection with human cytomegalovirus (HCMV) or serum treatment. Comparative limited proteolysis suggested that the [3H]glucosamine-labelled 130K polypeptides induced by these different stimuli were not identical. This observation was in contrast to results obtained by immunoprecipitation with antisera raised against the 130K glycopolypeptide from serum-induced cells which favoured a relatedness to the 130K polypeptide from virus infected cultures. Two-dimensional separation by isoelectric focusing and SDS-polyacrylamide gel electrophoresis subsequently showed that the 130K glycopolypeptide from serum-induced cells consists of two components, one of which is identical to the single component observed in samples from HCMV-infected cultures. Experiments on the effect of glycosylation inhibitors on DNA replication in HCMV-infected as well as in serum-induced cells support the view that the host-specific chromatin-associated glycopolypeptide may be involved in DNA replication in infected cells.

Keywords: cytomegalovirus, chromatin-associated glycopolypeptide

Received 6 June 1983; accepted 17 August 1983.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 1983 by the Society for General Microbiology.