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J Gen Virol 64 (1983), 2771-2776; DOI 10.1099/0022-1317-64-12-2771
© 1983 Society for General Microbiology
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The Genomes of Bovine Papillomaviruses Types 3 and 4 are Colinear

L. W. Coggins1, I. Hettich2, K. T. Smith1, A. A. Slater1, F. A. Roe1, H. Pfister3 and M. S. Campo1

1 Beatson Institute for Cancer Research, Wolfson Laboratory for Molecular Pathology, Garscube Estate, Bearsden, Glasgow G61 1BD, Scotland, U.K.
2 Institut für Virologie, Zentrum für Hygiene, Universität Freiburg, Hermann Herder Strasse 11, 7800 Freiburg, F.R.G.
and3 Institut für Klinische Virologie, der Universität Erlangen-Nürnberg, Loschgestrasse 7, 8520 Erlangen, F.R.G.

The 7.2 kb genomic DNA of bovine papillomavirus type 3 (BPV-3) was molecularly cloned using its unique EcoRI site, and the 7.3 kb genome of BPV-4 was cloned using its single BamHI site. The viral genomes were compared by liquid hybridization, Southern blot hybridization and heteroduplex mapping. Low stringency hybridization conditions revealed that the genomes are colinear but the sequences are extensively mismatched. The relative alignment of the restriction endonuclease maps of the two viral genomes has been determined. It was found that the genomes as linearized for cloning are out of phase by 1.7 kb, so that the single EcoRI site of BPV-3 appears to coincide with the BPV-4 EcoRI site at 0.22 map units. It is concluded that the genomes of BPV-3 and BPV-4, both of which cause true epithelial warts, share the same physical organization but exhibit sequence divergence.

Keywords: BPV, homology, genome

Received 28 June 1983; accepted 1 September 1983.




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