HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Taniguchi, K. Articles by Urasawa, T. Search for Related Content PubMed PubMed Citation Articles by Taniguchi, K. Articles by Urasawa, T. Agricola Articles by Taniguchi, K. Articles by Urasawa, T.

Monoclonal Antibodies Reactive with Different Sites of N and H Antigenic Particles of Poliovirus

Department of Hygiene, Sapporo Medical College, South-1, West-17, Chuo-ku, Sapporo 060, Japan

We established three hybridomas (designated 2A12, 4C4 and 6H8) secreting monoclonal antibodies which react with N and H(heated) antigenic particles of poliovirus type 1 (Mahoney strain). One of these monoclonal antibodies, 4C4, had virus-neutralizing activity, while the other two were non-neutralizing. A solid-phase radioimmunoassay and a neutralization test indicated that the specificity of the neutralizing monoclonal antibody 4C4 was the same as that of the monospecific antibody HN31, previously prepared by us by absorbing poliovirus antiserum with an equine serum inhibitor-resistant mutant (M-HN31) of poliovirus. Furthermore, by means of immune electron microscopy, it was revealed that the combining site of the 4C4 neutralizing monoclonal antibody is on or around vertices of the N and H antigenic particles, while the other two monoclonal antibodies are directed to the entire surface of the antigens.

Keywords: poliovirus, monoclonal antibody, H antigen, neutralization

Received 10 June 1983; accepted 23 August 1983.