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Cellular DNA Surrounding Integration Sites of an Avian Retrovirus

Renee Fitts

McArdle Laboratory, University of Wisconsin, Madison, Wisconsin 53706, U.S.A.

The size of the direct repeats of cellular DNA next to a spleen necrosis virus (SNV) provirus from infected rat cells and the nature of the cellular DNA surrounding SNV integration sites in chicken DNA were studied. A five-base pair repeat, ATTTT, was observed at the SNV-rat cell junctions. Five of ten SNV proviruses from chicken cells were flanked by unique DNA and five others were flanked by repetitive DNA. No large rearrangements of cellular DNA at SNV integration sites were observed. A clone of uninfected chicken DNA containing the presumptive unoccupied integration site for one SNV provirus was obtained and part of it was sequenced. A small substitution, approximately 60 nucleotides, was observed at the site of virus DNA insertion. Its significance is not known.

Keywords: spleen necrosis virus, direct repeat, unique DNA, DNA substitution

Present address: Integrated Genetics, 57 New York Ave., Framingham, Massachusetts 01701, U.S.A.

Received 4 May 1982; accepted 21 September 1982.