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J Gen Virol 64 (1983), 415-419; DOI 10.1099/0022-1317-64-2-415
© 1983 Society for General Microbiology
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Antiviral Effects of Human Fibroblast Interferon from Escherichia coli against Encephalomyocarditis Virus Infection of Squirrel Monkeys

Phillip K. Weck{dagger}, Richards N. Harkins and Nowell Stebbing1

Genentech, Inc., 460 Point San Bruno Blvd., South San Francisco, California 94080
and1 Applied Molecular Genetics, Inc. 1900 Oak Terrace Lane, Newbury Park, California 91320, U.S.A.

Recombinant DNA methodology has allowed the production of human fibroblast interferon (IFN-beta) from Escherichia coli and this material, in highly purified form, has been shown to reduce viraemia and mortality in encephalomyocarditis (EMC) virus-infected squirrel monkeys. These effects are dose related: six treatments over 4 days at 106 U/kg and 3 x 103 U/kg have comparable efficacy, whereas treatments at 103 U/kg are ineffective. The recombinant DNA-derived IFN-beta appears to be as effective as natural fibroblast cell-derived IFN-beta and both materials are effective by the intramuscular or intravenous routes. Thus, even though previous studies have shown that low circulating concentrations of IFN-beta are observed after intramuscular injections, the present data indicate that this slow release from the muscle can still confer protection.

Keywords: encephalomyocarditis virus, interferon (HuIFN-beta), E. coli, monkeys

{dagger} Present address: Department of Clinical Investigation, Burroughs Wellcome Co., 3030 Cornwallis Road, Research Triangle Park, N.C. 27709, U.S.A.

Received 13 July 1982; accepted 21 September 1982.




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Copyright © 1983 by the Society for General Microbiology.