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Studies on the Methylation of Avian Sarcoma Proviruses in Permissive and Non-permissive Cells

Department of Microbiology College of Physicians and Surgeons Columbia University, New York New York 10032, U.S.A.

The extent of methylation of several avian oncogenic proviruses was determined by using the restriction endonucleases HpaII and MspI. The results indicated that the transformation-defective proviruses (RAV-O or B77-td), which are exogenously introduced into avian host cells, were not methylated. However, endogenous proviruses (RAV-O) or ASV proviruses present in non-permissive host cells were found to be partly or completely methylated. The methyl-sensitive restriction endonuclease PvuI, which recognizes a unique site within the long terminal repeat in the ASV genome, failed to cleave proviruses present in several non-permissive host cells. From these results we suggest that modification of the sequence around the PvuI site results in reduced levels of transcription.

Keywords: avian oncogenic proviruses, methylation, transcription

Received 18 March 1982; accepted 21 September 1982.

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