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Replication of Influenza A and B Viruses in Human Diploid Cells

L. Herrero-Uribe^1,

¹ Department of Medical Microbiology London School of Hygiene & Tropical Medicine, Keppel Street, London WC1
and² Division of Virology National Institute for Biological Standards and Control, Holly Hill, Hampstead, London NW3, U.K.

Under optimal conditions, of high multiplicities of infection and with trypsin included in the medium throughout the incubation period, high yields of infectious influenza A and B viruses (10^6·5 p.f.u./ml) and of antigenically active haemagglutinin (HA) (1 µg/HA/10⁶ cells) were produced in human diploid MRC-5 cells. Budding virus particles were seen as spherical or short rod-like protrusions on the surface of the infected cells, and also on cell filopodia. Virus-induced cytoplasmic and nuclear inclusions were present in infected cells. This virus-human cell system may be suitable for studies of influenza virus persistence and for production of immunologically active HA antigen.

Keywords: influenza virus, diploid cells, antigens, replication

Present address: Instituto de Investigationes en Salud, University of Costa Rica, San Jose, Costa Rica.

Received 28 May 1982; accepted 20 September 1982.