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Oligonucleotide Fingerprinting of the RNA Species Obtained from Six Drosophila C Virus Isolates

Jonathan P. Clewley^1,

¹ Department of Biological Sciences University of Warwick, Coventry CV4 7AL
² NERC Institute of Virology, Mansfield Road, Oxford OX1 3SR, U.K.

The virion RNAs of six independent isolates of Drosophila C virus (DCV) have been characterized by ribonuclease T₁ oligonucleotide fingerprinting. All six isolates share common large oligonucleotides. Two of the isolates, from Vigier and Charolles, are closely related while a third French isolate from Gif is more distantly related. The other three isolates, two from Morocco (Taroudant and Ouarzazate) and one from the French Antilles were mixtures of more than one variant of DCV, but were clearly related to the three French isolates.

Keywords: picornaviruses, Drosophila, fingerprinting

Present address: Central Public Health Laboratory, Virus Reference Laboratory, 175 Colindale Avenue, London NW9 5HT, U.K.

Received 17 June 1982; accepted 20 August 1982.