HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Dutia, B. M. Search for Related Content PubMed PubMed Citation Articles by Dutia, B. M. Agricola Articles by Dutia, B. M.

Ribonucleotide Reductase Induced by Herpes Simplex Virus has a Virus-specified Constituent

Medical Research Council Virology Unit, Church Street, Glasgow G11 5JR, U.K.

Ribonucleotide reductase, an enzyme found in all prokaryotic and eukaryotic cells that synthesize DNA, is induced by herpes simplex virus (HSV). In this study the effect of anti-HSV antiserum on the induced ribonucleotide reductase has been examined and the ability of different temperature-sensitive (ts) mutants of HSV-1 to induce the enzyme has been investigated. The HSV-1-induced ribonucleotide reductase was inhibited by antiserum raised against infected cell lysates but not by preimmune serum. The wild-type (ts⁺) virus induced similar levels of ribonucleotide reductase at 31 °C and 38.5 °C (the permissive and non-permissive temperatures respectively for the ts mutants). All ts mutants induced approximately wild-type levels of the enzyme at 31 °C. At 38.5 °C, two of the four ts mutants studied also induced wild-type levels of enzyme but ts G failed to induce any activity while ts K induced variable but low levels. The enzyme activity induced by ts G at 31 °C was thermolabile both in vivo and in vitro. These results provide the first strong evidence that the induced ribonucleotide reductase activity is at least partially virus-coded.

Keywords: HSV, ribonucleotide reductase, ts mutants

Received 30 April 1982; accepted 1 October 1982.

This article has been cited by other articles:

				J. W. Nelson, J. Zhu, C. C. Smith, M. Kulka, and L. Aurelian ATP and SH3 Binding Sites in the Protein Kinase of the Large Subunit of Herpes Simplex Virus Type 2of Ribonucleotide Reductase (ICP10) J. Biol. Chem., July 19, 1996; 271(29): 17021 - 17027. [Abstract] [Full Text] [PDF]

				J. Trimble, S. Murthy, A Bakker, R Grassmann, and R. Desrosiers A gene for dihydrofolate reductase in a herpesvirus Science, March 4, 1988; 239(4844): 1145 - 1147. [Abstract] [PDF]