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Can Epstein—Barr Virus Infect and Transform All the B-Lymphocytes of Human Cord Blood?

M. Zerbini

Department of Tumor Biology, Karolinska Institutet, S-104 01 Stockholm, Sweden

Quantitative aspects of Epstein—Barr virus infection and transformation of human neonatal B-lymphocytes have been investigated. 72 to 90% B-cells were obtained with enrichment. Of the B-cells, 19 to 97% showed nuclear antigen (EBNA) 2 days after infection. A difference between different B-cell donors in susceptibility to infection was noted. Analysis of the virus dose—response curves obtained with twofold virus dilutions showed that one virus particle is sufficient to induce EBNA in a cell. Of the infected cells, 50 to 95% multiplied in microtitre wells containing a human fibroblast feeder layer, while only a small proportion established growing colonies in soft agarose, that could be picked up and subcultured.

Keywords: EBV, lymphocytes, EBNA

Present address: Institute of Microbiology, University of Bologna, Osp.S. Orsola, Via Massarenti 9, 40138 Bologna, Italy.

Received 6 July 1982; accepted 26 October 1982.

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