HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Schneider, J. Articles by Hunsmann, G. Search for Related Content PubMed PubMed Citation Articles by Schneider, J. Articles by Hunsmann, G. Agricola Articles by Schneider, J. Articles by Hunsmann, G.

Virosomes Constructed from Lipid and Purified Friend Leukaemia Virus Glycoprotein

¹ Forschergruppe Tumorimmunologie, Stefan-Meier-Strasse 8, D-7800 Freiburg
and² Institut für Biologie II der Universität, Zellbiologie, Schänzlestrasse 1, D-7800 Freiburg, Federal Republic of Germany

Liposomes were loaded by a dialysis technique with purified envelope glycopolypeptide, gp85, of the Friend murine leukaemia virus (F-MuLV). The gp85 liposomes prepared from cellular lipid had a buoyant density of 1.05 g/ml and an apparent diameter of 50 to 300 nm. The gp85 was not simply entrapped by liposomes nor adsorbed non-specifically to their outer surface. Experiments with radioactively labelled protein, electron microscopic examinations, protease treatment and concanavalin A binding showed that gp85 is anchored in the liposomal membrane and orientated asymmetrically as in the virus envelope. Moreover, gp85-covered liposomes displayed some functions of the intact F-MuLV envelope, such as absorption of antibodies to gp70 and haemagglutination after enzyme treatment. To some extent, these lipid vesicles appeared to be reconstituted F-MuLV envelopes and thus, by analogy to other systems, were named retrovirosomes.

Keywords: F-MuLV, liposomes, membrane glycoprotein, virosomes

Received 7 September 1982; accepted 1 November 1982.