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Purification, Identity and Some Properties of An Isolate of Barley Yellow Dwarf Virus from Indiana

Department of Botany and Plant Pathology, and Department of Entomology and USDA ARS, Purdue University, West Lafayette, Indiana 47907, U.S.A.

Methods were developed for preparing an Indiana isolate of barley yellow dwarf virus in amounts (1 to 2 mg/kg) and purity comparable to those recently achieved with other luteoviruses. Choice of host, and environmental conditions, period of infection and tissue used were critical factors for virus production. Aphid transmission, serological tests, and other properties defined the isolate as ‘PAV-like’ sensu Rochow, but the purification procedure worked well with ‘MAV’ and ‘RPV’, two other distinctive vector-specific isolates. The Indiana isolate had a particle diameter of 26 nm, s_20,w of approximately 115S, densities of 1.40 g/ml in CsCl and 1.335 g/ml in Cs₂SO₄, a single coat protein of mol. wt. 24400 (± 200) and an absorbance spectrum with an average A₂₆₀/A₂₈₀ ratio of 1.76.

Keywords: BYDV, purification and properties, luteovirus

Received 29 June 1982; accepted 25 October 1982.

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