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Intracellular RNA Expressed in Black Beetle Virus-infected Drosophila Cells

B. Reavy

NERC Institute of Virology, Mansfield Road Oxford OX1 3SR, U.K.

Black beetle virus replicates in Drosophila cells with the synthesis of virus-specific RNA and protein being detected as early as 2 h post-infection and continuing for at least 48 h. Shut-off of host synthesis did not occur in infected cells until 8 to 10 h post-infection. Examination of the RNA from infected cells on denaturing gels or on agarose/acrylamide gels demonstrated the production of four RNA species not found in uninfected cells. Two of these species, being single-stranded, co-migrated with the virion RNA and the other two were the double-stranded replicative stages of the virion RNA. No additional RNA species were found in infected cells, either in the presence or absence of actinomycin D.

Keywords: black beetle virus, Drosophila cells, RNA synthesis

Present address: Department of Genetics, Animal Virus Research Institute, Pirbright, Woking, Surrey GU24 0NF, U.K.

Received 10 September 1982; accepted 25 October 1982.