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Immunofluorescence Studies on the Antigenic Interrelationships of the Hughes Virus Serogroup (Genus Nairovirus) and Identification of a New Strain

London School of Hygiene and Tropical Medicine, Arbovirus Research Unit, Winches Farm Field Research Station, 395 Hatfield Road, St. Albans, Herts., U.K.

Titrations of hyperimmune antisera by indirect immunofluorescence using each virus of the Hughes serogroup (Hughes, Zirqa, Punta Salinas, Soldado and Farallon) demonstrated their individual antigenic identities. Furthermore, an antigenically related virus, designated Puffin Island (PI) virus, was shown both by indirect immunofluorescence and by neutralization in XTC cells to be distinguishable from the other viruses. These viruses readily established persistent infections in Vero cells after producing only moderate cytopathic effects. Treatment of persistently infected cultures with either fluorodeoxyuridine or bromodeoxyuridine made no significant difference to the percentage of immunofluorescent cells. Attempts to demonstrate haemagglutination by Zirqa virus were unsuccessful.

Keywords: Hughes serogroup, immunofluorescence, Nairovirus

Received 3 June 1982; accepted 11 November 1982.