J Gen Virol Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Gen Virol 64 (1983), 853-861; DOI 10.1099/0022-1317-64-4-853
© 1983 Society for General Microbiology
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hancock, K.
Right arrow Articles by Palmer, E. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hancock, K.
Right arrow Articles by Palmer, E. L.
Agricola
Right arrow Articles by Hancock, K.
Right arrow Articles by Palmer, E. L.

Adaptation of Two Avian Rotaviruses to Mammalian Cells and Characterization by Haemagglutination and RNA Electrophoresis

Kathy Hancock{dagger}, Gary G. William, Jr.{ddagger} and Erskine L. Palmer

Division of Viral Diseases, Center for Infectious Diseases, Centers for Disease Control, Public Health Service, U.S. Department of Health and Human Services, Atlanta, Georgia 30333, U.S.A.

Turkey and chicken rotaviruses were successfully adapted to replicate in a rhesus monkey embryo kidney (MA104) cell line. Trypsin treatment of virus and cells was essential for serial passage of the viruses. Polyacrylamide gel electrophoresis separated the viral RNA into 11 segments with clear resolution of segments 10 and 11. Segment 5 migrated with size class I, and this characteristic appeared to be a unique feature of the avian rotavirus genome. The ability of the avian rotaviruses to haemagglutinate a variety of erythrocytes was demonstrated. A type-specific antigen was detected by haemagglutination-inhibition assays and a group or subgroup antigen by enzyme immunoassays. Treatment of serum with heparin-MnCl2 was shown to be the best method for removing non-specific inhibitors of haemagglutination.

Keywords: avian rotavirus, haemagglutination, RNA analysis, cultivation

{dagger} Present address: Division of Parasitic Diseases, Center for Infectious Diseases, Centers for Disease Control, Atlanta, Ga. 30333, U.S.A.

{ddagger} Present address: Division of Hepatitis and Viral Enteritis, Centers for Disease Control, 4402 North 7th Street, Phoenix, Ariz. 85014, U.S.A.

Received 18 June 1982; accepted 16 November 1982.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 1983 by the Society for General Microbiology.