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Affinity Chromatography of Human Fibroblast Interferon (IFN-₁) by Monoclonal Antibody Columns

¹ Department of Virology
and² Department of Chemical Immunology, Weizmann Institute of Science, Rehovot, Israel

Eight hybridomas secreting monoclonal antibodies specific to human fibroblast interferon (IFN-₁) were identified by a new screening procedure based on immune precipitation with a second antibody and recovery of interferon activity from the immune precipitate. Immunoadsorbents were prepared from these monoclonal antibodies and used for purification of IFN-₁. Electrophoretically pure IFN-₁, having a specific activity of 3 x 10⁸ to 6 x 10⁸ units/mg was obtained by a single passage of partially purified IFN-₁ on these immunoadsorbents.

Keywords: interferon-, monoclonal antibody, affinity chromatography

Received 20 July 1982; accepted 22 October 1982.

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