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J Gen Virol 64 (1983), 921-926; DOI 10.1099/0022-1317-64-4-921
© 1983 Society for General Microbiology
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Isolation and Characterization of Host Range Mutants of Avian Sarcoma Virus

Shimakage Misuzu Iwasaki1, Masuo Yutsudo2, Akira Hakura2 and Kumao Toyoshima3

1 Department of Microbiology, Wakayama Medical College, Wakayama 640
2 Department of Tumor Virology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565
and3 Department of Oncology, The Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo 108, Japan

Two host range mutants of avian sarcoma virus (ASV) were isolated from a clone of the Bratislava 77 strain, subgroup C (B77-C). An HR- mutant was obtained by adaptation to duck cells and showed almost the same efficiencies of transformation (e.o.t.) and growth in cultures of chick embryo fibroblasts (CEF) and duck embryo fibroblasts (DEF). An HR+ mutant showed lower e.o.t. and slower growth on DEF than on CEF. Characterization of these mutants and wild-type B77-C showed that (i) unlike the host range mutants reported previously, these mutants and wild-type B77-C have common antigenicity, (ii) the number of infective centres is almost the same for the two mutants and the wild-type, and so the adsorption and integration of these viruses are similar, and (iii) the content of viral RNA in DEF infected with HR- is increased and decreased in the case of HR+ compared with the wild-type.

Keywords: ASV, host range, duck cells, RNA content of virus

Received 23 June 1982; accepted 2 December 1982.




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