| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Electron Microscopy Research Group, Section of Histopathology, Clinical Research Centre, Harrow, Middlesex, HA1 3UJ
and1 Division of Virology, National Institute of Medical Research, Mill Hill, London, U.K.
Scanning electron microscopy and immunofluorescence techniques show that the attachment of adenovirus type 5 to HeLa cells is followed by a temperature-dependent redistribution of virus particles on the cell surface. Metabolic inhibitors and cytochalasin B, a drug that impairs microfilament function, blocked this redistribution. Transmission electron microscopy studies demonstrated that inhibition of redistribution was paralleled by an inhibition of virus internalization. In further experiments virus and cells were incubated at 4 °C and then treated with adenovirus ‘soluble’ antigens to block unoccupied virus receptors. On warming these preparations to 37 °C, the internalization of attached virions was found to be impaired. It is proposed that energy-dependent redistribution of attached adenovirus particles on the cell surface facilitates entry by bringing virions into contact with sufficient receptors to allow internalization.
Keywords: virus entry, adenovirus, receptors, EM
Received 7 September 1982;
accepted 14 December 1982.
This article has been cited by other articles:
|
|
 |
|
  A. G. Dupuy and E. Caron Integrin-dependent phagocytosis - spreading from microadhesion to new concepts J. Cell Sci., June 1, 2008; 121(11): 1773 - 1783. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Egami, K. Ohuchida, K. Mizumoto, M. Onimaru, H. Toma, S. Nishio, E. Nagai, K. Matsumoto, T. Nakamura, and M. Tanaka Radiation Enhances Adenoviral Gene Therapy in Pancreatic Cancer via Activation of Cytomegalovirus Promoter and Increased Adenovirus Uptake Clin. Cancer Res., March 15, 2008; 14(6): 1859 - 1867. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Kawakami, H. Li, J. T. Lam, V. Krasnykh, D. T. Curiel, and J. L. Blackwell Substitution of the Adenovirus Serotype 5 Knob with a Serotype 3 Knob Enhances Multiple Steps in Virus Replication Cancer Res., March 15, 2003; 63(6): 1262 - 1269. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. J. D. Ashbourne Excoffon, T. Moninger, and J. Zabner The Coxsackie B Virus and Adenovirus Receptor Resides in a Distinct Membrane Microdomain J. Virol., February 15, 2003; 77(4): 2559 - 2567. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Richterova, D. Liebl, M. Horak, Z. Palkova, P. Hozak, J. Korb, and J. Forstova Caveolae Are Involved in the Trafficking of Mouse Polyomavirus Virions and Artificial VP1 Pseudocapsids toward Cell Nuclei J. Virol., November 15, 2001; 75(22): 10880 - 10891. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Sanlioglu, P. K. Benson, J. Yang, E. M. Atkinson, T. Reynolds, and J. F. Engelhardt Endocytosis and Nuclear Trafficking of Adeno-Associated Virus Type 2 Are Controlled by Rac1 and Phosphatidylinositol-3 Kinase Activation J. Virol., October 1, 2000; 74(19): 9184 - 9196. [Abstract] [Full Text]
|
|
|
|
|
|
M. Y. Nakano, K. Boucke, M. Suomalainen, R. P. Stidwill, and U. F. Greber The First Step of Adenovirus Type 2 Disassembly Occurs at the Cell Surface, Independently of Endocytosis and Escape to the Cytosol J. Virol., August 1, 2000; 74(15): 7085 - 7095. [Abstract] [Full Text]
|
|
|
|
 |
|
 E. Li, D. G. Stupack, S. L. Brown, R. Klemke, D. D. Schlaepfer, and G. R. Nemerow Association of p130CAS with Phosphatidylinositol-3-OH Kinase Mediates Adenovirus Cell Entry J. Biol. Chem., May 5, 2000; 275(19): 14729 - 14735. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. S. L. Parker and C. R. Parrish Cellular Uptake and Infection by Canine Parvovirus Involves Rapid Dynamin-Regulated Clathrin-Mediated Endocytosis, Followed by Slower Intracellular Trafficking J. Virol., February 15, 2000; 74(4): 1919 - 1930. [Abstract] [Full Text]
|
|
|
|
 |
|
 G. R. Nemerow and P. L. Stewart Role of alpha v Integrins in Adenovirus Cell Entry and Gene Delivery Microbiol. Mol. Biol. Rev., September 1, 1999; 63(3): 725 - 734. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Li, D. Stupack, G. M. Bokoch, and G. R. Nemerow Adenovirus Endocytosis Requires Actin Cytoskeleton Reorganization Mediated by Rho Family GTPases J. Virol., November 1, 1998; 72(11): 8806 - 8812. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Wang, S. Huang, A. Kapoor-Munshi, and G. Nemerow Adenovirus Internalization and Infection Require Dynamin J. Virol., April 1, 1998; 72(4): 3455 - 3458. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Li, D. Stupack, R. Klemke, D. A. Cheresh, and G. R. Nemerow Adenovirus Endocytosis via alpha v Integrins Requires Phosphoinositide-3-OH Kinase J. Virol., March 1, 1998; 72(3): 2055 - 2061. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
