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J Gen Virol 64 (1983), 1181-1186; DOI 10.1099/0022-1317-64-5-1181
© 1983 Society for General Microbiology
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Expression of Varicella-Zoster Virus-related Antigens in Biochemically Transformed Cells

Patricia Lopetegui, Yoshino Matsunaga, Toshiomi Okuno, Takeo Ogino1 and Koichi Yamanishi

Department of Virology, Research Institute for Microbial Diseases, Osaka University, Yamadaoka, Suita 565, Japan
and1 Department of Microbiology, Hiroshima City Institute of Public Health, 16-60, 4 Chome, Shoko Center Nishi-ku, Hiroshima City, Hiroshima 733, Japan

L(O)cl 3 and L(K)cl 1 cells, biochemically transformed by varicella-zoster virus (VZV), were labelled with L-[35S]methionine and [32P]orthophosphate. Cell extracts were immunoprecipitated with anti-VZV monkey serum and analysed by SDS-polyacrylamide gel electrophoresis. Four polypeptides of apparent mol. wt. 135000 (135K), 48K, 44K and 35K were detected in the L-[35S]methionine-labelled extracts, and, of these, the 35K band showed marked intensity. However, this band was not detected in extracts from cells infected with a VZV tk- strain (Kanno strain). Also, the 35K polypeptide showed very low intensity when immunoprecipitated from extracts of transformed cells grown in non-selective (NS) medium, i.e. cells that had a very low thymidine kinase (tk) activity. In the case of [32P]orthophosphate-labelled cells, polypeptides of apparent mol. wt. 180K, 81K, 48K, 44K and 37K were obtained. In both instances, the 44K polypeptide was not immunoprecipitated from L(K)cl 1 cell extracts. From our data it is postulated that the expression of the 35K polypeptide is correlated with the VZV-specific tk activity of the cells.

Keywords: varicella-zoster virus, transformation, thymidine kinase

Received 7 September 1982; accepted 15 December 1982.




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Copyright © 1983 by the Society for General Microbiology.